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Biochemical Society Transaction Cover image-August 2023
Our review on the role of cell-matrix and cell-cell interaction mechanics is featured on the cover. The image shows 2 immune cells squeezing through a dense network of tissue in a Xenopus embryo.
Bio-Protocol - April 2022 issue -
Front cover
My protocol describes a simple assay to measure and quantify the internalised dextran in cultured cells using Fiji macro.
Cancer Collection Special issue 2022
My paper on the role of CYRI-A, macropinocytosis and integrin trafficking was selected to be included in the Journal of Cell Biology Cancer Collection Special issue 2022 and is featured on the front cover. You can read more here: https://rupress.org/jcb/collection/21302/Cancer-Cell-Biology-2022
JCB - September 2021 issue -
Front cover
The main paper from my PhD describing the function of a novel protein CYRI-A and its involvement in macropinocytosis is featured on the front cover of the Journal of Cell Biology - September issue 2021. DOI: https://doi.org/10.1083/jcb.202012114
Structure - March 2021 issue -
Front cover
A collaborative project during my PhD on the structure of CYRI-B is featured on the front cover of the journal Structure. DOI: https://doi.org/10.1016/j.str.2020.11.003
My publications
HA Le, R Mayor., 2023. Cell–matrix and cell–cell interaction mechanics in guiding migration. Biochemical Society Transactions. - Featured on the journal cover - August 2023. DOI: https://doi.org/10.1042/BST20230211
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YELLAND, T.*, LE, A. H.*, NIKOLAOU, S., INSALL, R., MACHESKY, L. & ISMAIL, S. 2020. Structural Basis of CYRI-B Direct Competition with Scar/WAVE Complex for Rac1. Structure. (* co-first authors) – Featured on the journal cover - March 2021 issue. DOI: https://doi.org/10.1016/j.str.2020.11.003
In this paper, we describe the first co-crystal structure of the protein CYRI-B with its binding partner Rac1. We based on the similarity between CYRI-B and CYFIP to propose a more refined mechanism of how active Rac1 activates the Scar/WAVE complex. We also describe a novel dimerisation mechanism between CYRI molecules as a new regulation mechanism.
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LE, A. H., YELLAND, T., PAUL, N. R., FORT, L., NIKOLAOU, S., ISMAIL, S. & MACHESKY, L. M. 2020. CYRI-A limits invasive migration through macropinosome formation and integrin uptake regulation. Journal of Cell Biology - Featured on the journal cover - September 2021 issue. DOI: https://doi.org/10.1083/jcb.202012114
By using super-resolution microscopy, we describe the role of a novel protein called CYRI-A in helping resolve the formation of macropinosomes – structures cells use to uptake external substances. CYRI-A achieves this by locally suppressing active RAC1 signalling, therefore regulating how actin polymerises at the macropinocytic cups. By using different 2D and 3D invasion assays, we further show that CYRI protein family also involves in regulating surface integrins in cancer cells through macropinocytosis, and therefore controlling how cancer cells migrate and invade.
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LE, A. H., MACHESKY, L. M. 2022. Image-based Quantification of Macropinocytosis Using Dextran Uptake into Cultured Cells. Bio-Protocol - Featured on the journal cover - April 2022 issue. DOI: 10.21769/BioProtoc.4367
A simple protocol for measuring and quantifying the internalised dextran in cultured cells. Also featured step-by-step Fiji macro analysis and explanation for people with no coding background.