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Cells and Their Wonders

Your go-to source for all the science of the cell and the life of the scientist working with them

JCB September issue 2021 (6th Sep) cover image
At the heart of a cancer cell
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Motion and motionless.png
The fire from within.png
The explosion of vesicle dynamic
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Integrins and the dancing cell
Structure cover.jpg
BSCB 2021 3rd winner
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Biochemical Society Transaction Cover image-August 2023

Our review on the role of cell-matrix and cell-cell interaction mechanics is featured on the cover. The image shows 2 immune cells squeezing through a dense network of tissue in a Xenopus embryo.

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Bio-Protocol - April 2022 issue -

Front cover

My protocol describes a simple assay to measure and quantify the internalised dextran in cultured cells using macro Fiji.

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Cancer Collection Special issue 2022

My paper on the role of CYRI-A, macropinocytosis and integrin trafficking was selected to be included in the Journal of Cell Biology Cancer Collection Special issue 2022 and is featured on the front cover. You can read more here: https://rupress.org/jcb/collection/21302/Cancer-Cell-Biology-2022

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JCB - September 2021 issue -
Front cover

My CYRI-A paper is featured on the front cover of the Journal of Cell Biology - September issue 2021. DOI: https://doi.org/10.1083/jcb.202012114

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Structure - March 2021 issue -
Front cover

My collaborative work on the structure of CYRI-B is featured on the front cover of the journal Structure. DOI: https://doi.org/10.1016/j.str.2020.11.003

My publications

YELLAND, T.*, LE, A. H.*, NIKOLAOU, S., INSALL, R., MACHESKY, L. & ISMAIL, S. 2020. Structural Basis of CYRI-B Direct Competition with Scar/WAVE Complex for Rac1. Structure. (* co-first authors) – Featured on the journal cover - March 2021 issue. DOI: https://doi.org/10.1016/j.str.2020.11.003
In this paper, we describe the first co-crystal structure of the protein CYRI-B with its binding partner Rac1. We based on the similarity between CYRI-B and CYFIP to propose a more refined mechanism of how active Rac1 activates the Scar/WAVE complex. We also describe a novel dimerisation mechanism between CYRI molecules as a new regulation mechanism.

HA Le, R Mayor., 2023. Cell–matrix and cell–cell interaction mechanics in guiding migration. Biochemical Society Transactions. - Featured on the journal cover - August 2023. DOI: https://doi.org/10.1042/BST20230211

In this review, we discuss the role of cell-matrix and particularly cell-cell interaction mechanics in guiding cell migration. We draw in examples from various different model organisms to show that the effects from the cellular microenvironment are just as significant as that of the ECM on cellular behaviours. 

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LE, A. H., YELLAND, T., PAUL, N. R., FORT, L., NIKOLAOU, S., ISMAIL, S. & MACHESKY, L. M. 2020. CYRI-A limits invasive migration through macropinosome formation and integrin uptake regulation. Journal of Cell Biology - Featured on the journal cover - September 2021 issue. DOI: https://doi.org/10.1083/jcb.202012114

By using super-resolution microscopy, we describe the role of a novel protein called CYRI-A in helping resolve the formation of macropinosomes – structures cells use to uptake external substances. CYRI-A achieves this by locally suppressing active RAC1 signalling, therefore regulates how actin polymerises at the macropinocytic cups. By using different 2D and 3D invasion assays, we further show that CYRI protein family also involves in regulating surface integrins in cancer cells through macropinocytosis, and therefore controlling how cancer cells migrate and invade.

LE, A. H., MACHESKY, L. M. 2022. Image-based Quantification of Macropinocytosis Using Dextran Uptake into Cultured Cells. Bio-Protocol - Featured on the journal cover - April 2022 issue. 

DOI:   10.21769/BioProtoc.4367

A simple protocol for measuring and quantifying the internalised dextran in cultured cells. Also featured step-by-step macro Fiji analysis and explanation for people with no coding background. 

All free PDF files of my publications can be accessed using this Google Drive link: https://drive.google.com/drive/folders/1GaGzTICau-D0YA9gwpbUAUI8eiGryP41?usp=sharing

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